| Author | Comment | Last activity | Moderation | ||
|---|---|---|---|---|---|
| EllenHarri | "It is a very Useful post. " | Read more » | about 4 years ago | |||
| eustatic | "Just posting this, as complaints are more vital than ever: Scott Eustis scott@healthygulf.org 2:29 PM (1 minute ago) to Faye Monitoring Exerpt fr..." | Read more » | over 4 years ago | |||
| stoft | "BTW .... the comments about LED 'voltage' perspective do not seem inline with LED parameters. With LEDs, it's not the voltage, it's the current you..." | Read more » | almost 9 years ago | |||
| warren | "Thanks, Dave, good to know. I'd sort of imagine it's a monochromator, like a reverse-spectrometer, using masking and a diffraction grating to gener..." | Read more » | almost 9 years ago | |||
| stoft | "Note: Their UV source was a PTI LS-100 fluorescence spectrophotometer -- no longer sold. From a google books print, it sounds like a variable-wavel..." | Read more » | almost 9 years ago | |||
| warren | "Ah, thanks for the download tip. OK, that's really interesting to see that lower excitation wavelengths create more variable/distinct emission spe..." | Read more » | almost 9 years ago | |||
| stoft | "Note: The article can be downloaded w/o signing up using the 'Download Full Text' button at the upper right of the image frame on the web page. Fr..." | Read more » | almost 9 years ago | |||
| warren | "Another thing that 1995 paper could tell us is why they chose 30nm as the offset to discard above the excitation wavelength, and more about the lig..." | Read more » | almost 9 years ago | |||
| warren | "I think @gretchengehrke is back from GOSH in Switzerland, so maybe she can chime in, but I wanted to question specifically @ethanbass's point: the..." | Read more » | almost 9 years ago | |||
| warren | "Gretchen is a chemist, so I thought she could shed some light on our questions about overlap and shorter wavelength LEDs. " | Read more » | almost 9 years ago | |||
| warren | "@gretchengehrke, can you help us w excitation emission wavelength questions? " | Read more » | almost 9 years ago | |||
| ethanbass | "One more thing to consider is that our DVD diffraction gratings would probably filter out most of the UV light below about 390 nm, even if we had a..." | Read more » | almost 9 years ago | |||
| stoft | "Aha; a very good description which explains things I'd read. I think it also directly supports my present contention that with a 400nm source and t..." | Read more » | almost 9 years ago | |||
| ethanbass | "@warren - According to the paper that figure is lifted from, you will get increasing fluorescence intensity (or quantum yield), as you increase the..." | Read more » | almost 9 years ago | |||
| ethanbass | "@warren & @stoft - It's important to remember that the range of fluorescent emissions is determined in large part by the excitation wavelength ..." | Read more » | almost 9 years ago | |||
| stoft | "RIght, Jeff, those are good questions and I'm not a Chemist -- I'm just extracting from what I glean from the web. But I think it would be good to ..." | Read more » | almost 9 years ago | |||
| stoft | "https://publiclab.org/profile/ethanbass - Thanks for your comments. As to your last note about my final plot: What I see is the depth of the "notch..." | Read more » | almost 9 years ago | |||
| warren | "Oh my goodness, I realized that I have always assumed that fluorescence emission wavelength was relative to something inherent in the chemical stru..." | Read more » | almost 9 years ago | |||
| stoft | "Ok, good points and questions. [ I do not intend to sound abrupt. I'm just focused on raising technical issues I see within and surrounding this t..." | Read more » | almost 9 years ago | |||
| ethanbass | "@warren - I don't think using a 395 nm LED or an even shorter wavelength is really going to solve the problem of the overlap between the light scat..." | Read more » | almost 9 years ago | |||
| warren | "Hi, Dave - thanks as always for a thorough response. Some comments: However, the wide bandwidth of the LED, as opposed to the laser, is clearly a ..." | Read more » | almost 9 years ago | |||
| ethanbass | "@warren - Please do send one along. I'd be happy to do some comparisons. " | Read more » | almost 9 years ago | |||
| warren | "Oh, did @matej end up with those? " | Read more » | almost 9 years ago | |||
| liz | "@warren - i do not have any of the polystyrene cuvettes " | Read more » | almost 9 years ago |