otk
otk


Author Comment Last activity Moderation
EllenHarri "It is a very Useful post. " | Read more » almost 2 years ago
eustatic "Just posting this, as complaints are more vital than ever: Scott Eustis scott@healthygulf.org 2:29 PM (1 minute ago) to Faye Monitoring Exerpt fr..." | Read more » over 2 years ago
stoft "BTW .... the comments about LED 'voltage' perspective do not seem inline with LED parameters. With LEDs, it's not the voltage, it's the current you..." | Read more » over 6 years ago
warren "Thanks, Dave, good to know. I'd sort of imagine it's a monochromator, like a reverse-spectrometer, using masking and a diffraction grating to gener..." | Read more » over 6 years ago
stoft "Note: Their UV source was a PTI LS-100 fluorescence spectrophotometer -- no longer sold. From a google books print, it sounds like a variable-wavel..." | Read more » over 6 years ago
warren "Ah, thanks for the download tip. OK, that's really interesting to see that lower excitation wavelengths create more variable/distinct emission spe..." | Read more » over 6 years ago
stoft "Note: The article can be downloaded w/o signing up using the 'Download Full Text' button at the upper right of the image frame on the web page. Fr..." | Read more » over 6 years ago
warren "Another thing that 1995 paper could tell us is why they chose 30nm as the offset to discard above the excitation wavelength, and more about the lig..." | Read more » over 6 years ago
warren "I think @gretchengehrke is back from GOSH in Switzerland, so maybe she can chime in, but I wanted to question specifically @ethanbass's point: the..." | Read more » over 6 years ago
warren "Gretchen is a chemist, so I thought she could shed some light on our questions about overlap and shorter wavelength LEDs. " | Read more » over 6 years ago
warren "@gretchengehrke, can you help us w excitation emission wavelength questions? " | Read more » over 6 years ago
ethanbass "One more thing to consider is that our DVD diffraction gratings would probably filter out most of the UV light below about 390 nm, even if we had a..." | Read more » over 6 years ago
stoft "Aha; a very good description which explains things I'd read. I think it also directly supports my present contention that with a 400nm source and t..." | Read more » over 6 years ago
ethanbass "@warren - According to the paper that figure is lifted from, you will get increasing fluorescence intensity (or quantum yield), as you increase the..." | Read more » over 6 years ago
ethanbass "@warren & @stoft - It's important to remember that the range of fluorescent emissions is determined in large part by the excitation wavelength ..." | Read more » over 6 years ago
stoft "RIght, Jeff, those are good questions and I'm not a Chemist -- I'm just extracting from what I glean from the web. But I think it would be good to ..." | Read more » over 6 years ago
stoft "https://publiclab.org/profile/ethanbass - Thanks for your comments. As to your last note about my final plot: What I see is the depth of the "notch..." | Read more » over 6 years ago
warren "Oh my goodness, I realized that I have always assumed that fluorescence emission wavelength was relative to something inherent in the chemical stru..." | Read more » over 6 years ago
stoft "Ok, good points and questions. [ I do not intend to sound abrupt. I'm just focused on raising technical issues I see within and surrounding this t..." | Read more » over 6 years ago
ethanbass "@warren - I don't think using a 395 nm LED or an even shorter wavelength is really going to solve the problem of the overlap between the light scat..." | Read more » over 6 years ago
warren "Hi, Dave - thanks as always for a thorough response. Some comments: However, the wide bandwidth of the LED, as opposed to the laser, is clearly a ..." | Read more » over 6 years ago
ethanbass "@warren - Please do send one along. I'd be happy to do some comparisons. " | Read more » over 6 years ago
warren "Oh, did @matej end up with those? " | Read more » over 6 years ago
liz "@warren - i do not have any of the polystyrene cuvettes " | Read more » over 6 years ago