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Spectrometry Sampling


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How do you test liquid or solid samples with your DIY Spectrometer? Read about ways to prepare and scan samples here, and read about different tests you can do with your spectrometer.

Questions

Title Author Updated Likes
Alternative spectrometry sample cells for Hach DR900 @fieldlab over 1 year ago
Testing Water For Specific Polluting Compounds @rajanzaveri over 1 year ago
What are good containers to use for spectrometry samples? @warren about 2 years ago
Mini spectrophotometer - how does this deviate from the Beer-Lambert law? @FoxClass over 2 years ago
Sample container search @warren over 4 years ago

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Sample containers

What do you store liquid samples in? A good sample container has flat sides, so you can shine lights (and lasers) through it without lots of reflections.

There are lots of different affordable test tubes on Amazon, but they're mostly round. Flat-sided test tubes are often called cuvettes and are specifically for spectrometry.

It's also good to have the light travel through a consistent amount of the sample -- many cuvettes (traditional spectrometry sample containers) are 1cm x 1cm, so the light always goes through 1cm of the sample.

dropper.jpg Cuvette_with_penny.jpg

A square-sided bottle, left, and a cuvette, right (photo from Wikipedia).

Unfortunately, we've found that cuvettes with plastic stoppers will leak when filled with oil and not kept upright (for example if you travel with them). A really nice source of completely sealable rectangular 1/4 oz glass jars which are pretty perfect for sampling can be found for $3.50 for a ten-pack here (see image below of 2 on top of a flashlight):

http://www.sciplus.com/p/WHITCAP-BOTTLE_48212

Other sample container options


Water sampling

Water is usually very clear in small amounts -- even murky water in a small container will look pretty transparent. That makes it hard to measure with spectrometry unless you shine light through a lot of it. But some tests have been done -- see this example of a scan of water from the Charles River before and after 7 days of settling, by Jeff Hecht:

charles-river.png


Oil sampling

However, most research in Public Lab to date has focused on oil spectroscopy -- attempting to identify petroleum residue in sediments.

IMG_20140722_230007_2.jpg

To identify oil contamination, we have been attempting to illuminate oil samples with UV flashlights and green lasers, which can make some oils fluoresce, or glow, as pictured above.

The basics of sample preparation for oil identification are still being refined, but our best practices to date can be found on the Oil Testing Kit page


Flame spectroscopy

Burning potato chips to measure the sodium emission spectrum from the NaCl (salt).

Flame spectroscopy

Another type of spectrometry which involves measuring the light of a flame and can detect specific elements (not molecules) as they emit light at very specific "peaks" -- narrow wavelength bands. Besides flames, these "emission lines" can be produced by exposing gases or sometimes liquids to UV light, lasers, or electric fields (as in a fluorescent bulb). The fluorescent bulb spectrum you get when calibrating is an example of a mercury emission spectrum.

Emission lines are produced by atoms, not whole molecules (the latter produce absorption lines, which we might still be able to detect since we have the flame -- a good broad-spectrum light source -- but that is just a theory at this point). So sulfur and carbon are possible targets, but we won't be able to distinguish CO2 from CO.

Basic setup: For a more complete description, please read about the "flare spectroscopy activity" below, however, the basic setup involves simply pointing a spectrometer at a flame (which can be difficult to line up if the flame is far away), and later comparing any peaks to known peak locations of looked-for elements. We are compiling a collection of such known elements by importing "idealized" spectra from the NIST database, a process which you can read more about here.

Read more about flame spectroscopy:


Activities

Purpose Category Status Author Time Difficulty Replications
Detecting Pesticides in Organic and Conventional Raspberries using Open Source Instrumentation - - @silverhammer - hard 0 replications: Try it »
First "PIG Oil Only Absorbent Mat Pad" Test - - @Matej - - 0 replications: Try it »
Separating Oil from Water by Freezing the Water - - @Matej - - 0 replications: Try it »
Preliminary LED and isopropyl alcohol solvent tests for oil fluorescence analysis - - @warren - - 0 replications: Try it »
Using the Spectroscope for Analysis of Concentration (Beer's Law) test-limits - @straylight 3h medium 0 replications: Try it »
Oil Testing Beta --plus Grand Isle tar - - @eustatic - - 0 replications: Try it »
Testing the Oil Testing Kit: identifying a "somewhat unknown" sample - - @warren - - 0 replications: Try it »
Spectrometry Sample Extraction at the Parts & Crafts Toolshed-Raising - - @warren - - 0 replications: Try it »
Spectrometer 3.0 add-on v2 - - @programmer1200 - - 0 replications: Try it »
Mobile cuvette holder - - @cfastie - - 0 replications: Try it »
Testing high-brightness 405nm LEDs in fluorescence spectroscopy of oils - - @warren - - 0 replications: Try it »
Honlian 60s LED nail polish hardener, various LED fluorescence updates - - @warren - - 0 replications: Try it »
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