Question: Which objectives do you use?

aquiles asked on July 13, 2018 07:34
91 | 1 answers | #16711


i loved the microscope kit!

I am trying to build cheap fluorescence microscopes and was wondering which objectives are you using and what is your experience with them.

Where did you source them?

Keep up with the amazing work!



4 Comments

Hi @aquilies just to add what @asnow said, we've been stacking the amscope lenses with Raspberry Pi cameras, which has worked well, but this is a new/evolving project, so I am sure as more people start to build their own microscopes we'll have more to share about the pros/cons of different types of lenses. You might also be interested in @MaggPi 's work using the microscope with modifications for infrared. The basic microscope actually uses an inverted webcam lens without an additional objective lens, with some impressive results.

I'd be interested to hear more about your work, do you have links to your project anywhere?

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@bronwen I would say that the second idea is better. Friend of mine have also been using amscope lenses with Raspberry Pi cameras and it hasn't worked good for him. I don't really remember what happened, but I'm sure he wasn't happy with this solution.


@bronwen, I don't have any links yet, just working on ideas in the lab while trying to find a good compromise quality/price. Coming from a 15k€ objective, I wonder how severe it would be the switch to the AmScope.


Hi all, Here is what I can share on Raspberry Pi and microscope objectives:

---I have worked on three different DIY microscope set ups:

-Public lab community microscope https://publiclab.org/notes/bronwen/05-07-2018/community-microscope-assembly-instructions (SPECIAL THANKS to @cfastie for help with the 3D printed camera/objective lens holder!!!!! https://publiclab.org/notes/partsandcrafts/02-15-2018/2-attaching-your-raspberry-pi-camera-to-a-microscope-objective-lens

-Modular lens mount system https://publiclab.org/notes/MaggPi/04-08-2018/raspberry-pi-microscope-close-up-lens-system

-substituting a raspberry pi camera in an old Celestron microscope.
This video shows a typical rebuild but I use a camera holder that was 3d printed : https://www.youtube.com/watch?v=M6TvCOPN5pI

---All of the systems above work equally well with Amscope or Celestron (standard) microscope objectives/ Raspberry PI cameras but there are some trade-offs: 1) Using objectives in non-standard configurations can reduce performance. An example is the use of a 30mm vs 160mm (standard) optical tubelength (see below).

2) Since the DIY microscope systems typically requires removing the factory installed Raspberry Pi lens, it is important to make sure dust does not reach the detector. If dust lands on the detector the choices are living with picture defects or risk damaging the detector while cleaning.

3)While objective lenses that I have used were built for visible light microscopy, the lenses generally work in the near infrared (with refocus) but not very well below 400nm. You can see some near infrared imagery here: https://publiclab.org/notes/MaggPi/06-21-2018/infrared-insect-microscopy Note I have tried 380nm diodes with my objectives but no light reaches the detector. Since I know the Raspberry PI camera has a 380nm UV response ( see: https://publiclab.org/notes/MaggPi/06-17-2018/how-can-you-use-computer-vision-to-reduce-spectral-overlap ) the glass used in the objective lens seems to be opaque to UV.

Examples below show the difference in magnification for the same camera (Raspberry Pi NoIR) and microscope objective (Celestron 20x) but with different tubelengths (30 vs 160mm). The image is of human blood cells which is a good reference target since the cells have about an 8 micron diameter and a 1 micron center diameter. The first slide shows human blood cells with a 30mm tubelength, 20x objective at two resolutions.

Slide1.JPG

The second slide show that the 160mm tube length (with the same objective) provides increased magnification(left picture). The right picture shows that magnification with 10x objective/160mm tubelength is greater than a 20xobjective/30mm tubelength. The difference in magnification between 30 and 160 mm tubelengths seems to be a least 5 times and may indicate that the magnification is directly proportional.

Slide2.JPG

So, I know I haven’t really answered the question about a low cost fluorescence microscope but I suspect any DIY design will have to compensate for using cheap optics. One possible compensation method may be computer vision software which can be designed to correct for image distortions.
Microscopy U offers a great explanation of specialized microscope objectives: https://www.microscopyu.com/microscopy-basics/specialized-microscope-objectives

@warren, @icarito, @amirberAgain

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1 Answers

Hi @acuiles! We typically source lenses from Amscope, they usually have the best price, free and fast shipping! We typically work with 10 or 20x. Amscope also has a variety 4-pack that you could consider, and last time I checked it was slightly cheaper on Amazon. Keep up the awesome work, we'd love to see what you create!


Thanks @asnow! I will try the amscope lenses to see how they perform. When I have some feedback, I'll post it here!


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