🎈 Public Lab: Revisions for 'Evaluating the Success of Mine Reclamation'

Revisions for Evaluating the Success of Mine Reclamation

30 CURRENT	laurel_mire	March 18, 2022 13:43 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Background info: Soil health as an indicator of reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag More on evaluating mine reclamation from Public Lab contributors [notes:grid:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. "Pine (slash pine) mycorrhizae 6 Sep 1964" by Plant pests and diseases, CC0 1.0. What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities This metric is calculated by counting the number of propagules. “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
29	laurel_mire	March 18, 2022 13:43 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Background info: Soil health as an indicator of reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag More on evaluating mine reclamation from Public Lab contributors [notes:grid:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. "Pine (slash pine) mycorrhizae 6 Sep 1964" by Plant pests and diseases, CC0 1.0. What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities This metric is calculated by counting the number of propagules. “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
28	laurel_mire	March 16, 2022 17:52 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Background info: Soil health as an indicator of reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag More on evaluating mine reclamation from Public Lab contributors [notes:grid:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities This metric is calculated by counting the number of propagules. “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
27	laurel_mire	March 14, 2022 17:40 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Background info: Soil health as an indicator of reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag More on evaluating mine reclamation from Public Lab contributors [notes:grid:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities This metric is calculated by counting the number of propagules. “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
26	laurel_mire	March 14, 2022 17:37 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Background info: Soil health as an indicator of reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag [notes:grid:evaluating-reclamation] Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities This metric is calculated by counting the number of propagules. “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
25	laurel_mire	March 07, 2022 19:03 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Background info: Soil health as an indicator of reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities This metric is calculated by counting the number of propagules. “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
24	laurel_mire	March 07, 2022 19:00 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Background info: Soil health as an indicator of reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities This metric is calculated by counting the number of propagules. “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
23	laurel_mire	March 07, 2022 18:58 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Background info: Soil health as an indicator of reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) - This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) - It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities - This metric is calculated by counting the number of propagules. * “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
22	laurel_mire	March 07, 2022 18:55 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Background info: Soil health as an indicator of reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) - This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) - It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities - This metric is calculated by counting the number of propagules. * “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme ). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
21	laurel_mire	March 07, 2022 18:53 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Background info: Soil health as an indicator of reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) - This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) - It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities - This metric is calculated by counting the number of propagules. * “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme ). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: - Measurement of dehydrogenase activity in acid soils rich in organic matter - Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
20	laurel_mire	March 07, 2022 18:52 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Background info: Soil health as an indicator of reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activitiy Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) - This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) - It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities - This metric is calculated by counting the number of propagules. * “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme ). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: - Measurement of dehydrogenase activity in acid soils rich in organic matter - Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
19	laurel_mire	March 07, 2022 18:40 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) - This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) - It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities - This metric is calculated by counting the number of propagules. * “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme ). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: - Measurement of dehydrogenase activity in acid soils rich in organic matter - Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
18	laurel_mire	March 07, 2022 18:39 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) - This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) - It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities - This metric is calculated by counting the number of propagules. * “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme ). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: - Measurement of dehydrogenase activity in acid soils rich in organic matter - Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
17	laurel_mire	March 07, 2022 18:10 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) - This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) - It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities - This metric is calculated by counting the number of propagules. * “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme ). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: - Measurement of dehydrogenase activity in acid soils rich in organic matter - Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
16	laurel_mire	March 07, 2022 18:06 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) - This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) - It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities - This metric is calculated by counting the number of propagules. * “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme ). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: - Measurement of dehydrogenase activity in acid soils rich in organic matter - Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
15	laurel_mire	March 07, 2022 18:05 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) - This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) - It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities - This metric is calculated by counting the number of propagules. * “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme ). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: - Measurement of dehydrogenase activity in acid soils rich in organic matter - Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
14	laurel_mire	March 07, 2022 18:05 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) - This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) - It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities - This metric is calculated by counting the number of propagules. * “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme ). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: - Measurement of dehydrogenase activity in acid soils rich in organic matter - Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
13	laurel_mire	March 07, 2022 18:04 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) - This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) - It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities - This metric is calculated by counting the number of propagules. * “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme ). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: - Measurement of dehydrogenase activity in acid soils rich in organic matter - Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
12	laurel_mire	March 07, 2022 18:03 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) - This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) - It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities - This metric is calculated by counting the number of propagules. * “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme ). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: - Measurement of dehydrogenase activity in acid soils rich in organic matter - Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert
11	laurel_mire	March 07, 2022 18:02 \| about 2 years ago Lead image: Abandoned coal mine subsidence stabilization project by BuzzWeiser196, CC-BY-SA-4.0 What’s on this page: Questions from the Public Lab community about evaluating mine reclamation success Activities on evaluating mine reclamation success Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi Indirect methods: mycorrhizal inoculum potential & most probable numbers estimate Direct method: mycorrhizal inoculum potential Measurement of dehydrogenase activity Improving soil health Resources and further reading Please help these resources grow by editing this page or adding and answering questions! You can also follow along and get updates on new resources by subscribing to the 'evaluating-reclamation' tag Questions about evaluating mine reclamation success Questions tagged with `question:evaluating-reclamation` will appear here [questions:evaluating-reclamation] Methods and activities on evaluating mine reclamation success Activities tagged with `activity:evaluating-reclamation` will appear here [notes:activity:evaluating-reclamation] Soil Health as an Indicator of Reclamation Success At its core, reclamation is an effort to restore harm done to a mined land’s soil health. Damage to soil from mining may include: altering microbial communities, changing soil horizon and structure, destruction of vegetation, upsetting nutrient cycling, & changing soil pH. After a reclamation plan has been carried out, it is important to evaluate how successful reclamation efforts have been. This evaluation can help inform future reclamation plans, help communities take action against a mining company, & formulate next steps as needed to return soils to a healthy state. Oftentimes, revegetation of mined areas is seen as successful reclamation. While revegetation is a useful tool for preventing soil erosion and encouraging soil health, it is not the end all be all. When evaluating the quality of a reclamation project, simply seeing plant life on the site is not enough to deem a reclamation effort successful. Several other factors are at play: is the vegetation native and can it thrive without human intervention, is the soil healthy (too acidic, are nutrient cycles reestablished, is the incredibly important microbial community functioning and have mycorrhizal relationships been reestablished?). Instead, a broader ecosystem perspective and analysis of soil health will provide a much more useful understanding of the state of the land, the quality of reclamation efforts, and the future viability & productivity of the reclaimed area. From an ecosystem perspective, success of reclamation can be assessed by comparing previously mined land to similar undisturbed sites. From this holistic perspective, there is no one metric that may deem a reclaimed site “good” or “bad.” Instead, “several parameters must be considered in order to determine the state and functionality of the soil system” (Soil Reclamation of Abandoned Mine Land by Revegetation: A Review). These parameters play into overall soil health and include metrics such as nutrient availability, the presence of microbial life and bacteria, and soil aggregation. The sections below give an overview of methods specifically used for determining soil health of reclaimed lands. However, any methodology testing an aspect of a soil’s health (such as these resources on soil-contaminant-testing & soil-sampling) will give a good indication of reclamation success. Methods of evaluating reclamation success through soil health Bioassays of soils for mycorrhizal fungi What is a mycorrhiza? A mycorrhiza is a “mutualistic association between plants and fungi” (source). Mycorrhizal fungi (fungi that participate in this association with plants) live in soil and grow on plant roots. The relationship benefits both the fungi and the plant: the fungus cannot fix its own carbon, so it instead gets this crucial element from the plant via the roots; the fungus returns the favor by absorbing nutrients from the soil that the plant (due to its shallower and thicker roots) cannot obtain itself. Passing these nutrients onto the plant via the roots, the mycorrhizal fungi improve the growth and fitness of the individual plant. Rather than assuming reclamation success by the simple presence of vegetation, testing soil for mycorrhizal activity offers a good indication of the health of the plants present and the viability of continued soil and vegetation health. Presence of mycorrhizal fungi signals healthy soil and successful reclamation efforts while lack thereof may necessitate further intervention. Image courtesy of Why and How to Improve Grass Roots with Mycorrhizal Fungi What is a bioassay? In general, a bioassay is a method that uses plants, animals, or other living things to measure the harmful effects of environmental pollution. For more info on bioassays, check out this wiki. In this case, growth of a mycorrhizal plant (a plant that will form an association with mycorrhizal fungi) is used as an indication of the presence of mycorrhizal fungi in the soil. This methodology can be used either indirectly (prior to revegetation, a test sample of soil is taken) or directly (plants from reclaimed land are collected). The indirect method may be useful for planning revegetation efforts or making the case that soil health is subpar, revegetation will not be viable, and other efforts to improve soil health must be taken. Direct testing is useful when revegetation is already underway (perhaps by the mining company itself) and you want to know more about soil quality and how plant life may fare in the long run. Indirect method A known amount of test soil is collected and mixed with standard, sterile soil. This mixture is then seeded with a mycorrhizal plant and left to grow. After a known period of time, the plant is harvested and 2 different metrics may be used to assess mycorrhizal activity: 1) Calculate the mycorrhizal inoculum potential (MIP) - This metric is estimated by the percentage of root tips infected by ectomcorrhizae or the percentage of root length infected by VA mycorrhizal (just depends on what type of mycorrhizal fungi is present) - It is a measure of the potential for mycorrhizal relationship between vegetation and fungi to reestablish after a disturbance to soil (like mining) 2) Calculate the most probable numbers estimate (MPN) of mycorrhizal fungi densities - This metric is calculated by counting the number of propagules. * “Propagules are an average count of colony-forming spores, hyphae, and root fragments per gram” (What are Mycorrhizal Propagules?). Direct method The direct method is carried out in the same manner as the indirect, just using plants taken directly from the reclamation site. At given time intervals, plants are collected from the revegetation/reclamation site and the MIP is calculated (#1 above) Measurement of dehydrogenase activity Dehydrogenase is a soil enzyme & has been used as a sensitive indicator of soil quality in degraded soils like that of previously mined areas. Soil enzymes speed up the decomposition of plant residue and the recycling of important nutrients like nitrogen, phosphorus, and carbon (Importance Of Soil Enzymes & Classes Of Soil Enzyme ). Soil enzymes respond to changes in the soil long before other soil quality indicators will be detectable, so measuring dehydrogenase is a great method to pick up on problems in the soil of reclaimed mining land early. More information on this method may be found here: - Measurement of dehydrogenase activity in acid soils rich in organic matter - Dehydrogenase Activity in the Soil Environment Improving Soil Health If you do find that reclamation efforts have been inadequate, there are a number of natural ways to improve soil health of previously mined land: - Add sawdust, wood residue, sewage sludge, animal manure to increase microbial activity Plant nitrogen-fixing species like legumes, field peas, & red clovers to increase soil fertility (source and more reading) Improve carbon sequestration Plant metal tolerant and pH adjusting plants (such as Gravellia robusta) if soil is found to have heavy metal contamination or pH has dropped Applying cement kiln dust or limestone is also effective for increasing soil pH Consider inoculating the soil with mycorrhizae propagules yourself Resources & Further Reading Reclamation in general Soil Reclamation of Abandoned Mine Land by Revegetation: A Review Plants That Enrich Your Garden Methods Mycorrhizal Activity Mycorrhizae – Wisconsin Horticulture The Use of Mycorrhizae in Mined Land Reclamation What Are Mycorrhizal Propagules? Soil Enzymes & Microbes Importance of Soil Enzymes Measurement of dehydrogenase activity in acid soils rich in organic matter Dehydrogenase Activity in the Soil environment	Revert

« »

Public Lab Wiki documentation

Revisions for Evaluating the Success of Mine Reclamation

More on evaluating mine reclamation from Public Lab contributors

Soil Health as an Indicator of Reclamation Success

Methods of evaluating reclamation success through soil health

Bioassays of soils for mycorrhizal fungi

Indirect method

Direct method

Measurement of dehydrogenase activity

Improving Soil Health

Resources & Further Reading

More on evaluating mine reclamation from Public Lab contributors

Soil Health as an Indicator of Reclamation Success

Methods of evaluating reclamation success through soil health

Bioassays of soils for mycorrhizal fungi

Indirect method

Direct method

Measurement of dehydrogenase activity

Improving Soil Health

Resources & Further Reading

More on evaluating mine reclamation from Public Lab contributors

Soil Health as an Indicator of Reclamation Success

Methods of evaluating reclamation success through soil health

Bioassays of soils for mycorrhizal fungi

Indirect method

Direct method

Measurement of dehydrogenase activity

Improving Soil Health

Resources & Further Reading

More on evaluating mine reclamation from Public Lab contributors

Soil Health as an Indicator of Reclamation Success

Methods of evaluating reclamation success through soil health

Bioassays of soils for mycorrhizal fungi

Indirect method

Direct method

Measurement of dehydrogenase activity

Improving Soil Health

Resources & Further Reading

Questions about evaluating mine reclamation success

Methods and activities on evaluating mine reclamation success

Soil Health as an Indicator of Reclamation Success

Methods of evaluating reclamation success through soil health

Bioassays of soils for mycorrhizal fungi

Indirect method

Direct method

Measurement of dehydrogenase activity

Improving Soil Health

Resources & Further Reading

Questions about evaluating mine reclamation success

Methods and activities on evaluating mine reclamation success

Soil Health as an Indicator of Reclamation Success

Methods of evaluating reclamation success through soil health

Bioassays of soils for mycorrhizal fungi

Indirect method

Direct method

Measurement of dehydrogenase activity

Improving Soil Health

Resources & Further Reading

Questions about evaluating mine reclamation success

Methods and activities on evaluating mine reclamation success

Soil Health as an Indicator of Reclamation Success

Methods of evaluating reclamation success through soil health

Bioassays of soils for mycorrhizal fungi

Indirect method

Direct method

Measurement of dehydrogenase activity

Improving Soil Health

Resources & Further Reading

Questions about evaluating mine reclamation success

Methods and activities on evaluating mine reclamation success

Soil Health as an Indicator of Reclamation Success

Methods of evaluating reclamation success through soil health

Bioassays of soils for mycorrhizal fungi

Indirect method

Direct method

Measurement of dehydrogenase activity

Improving Soil Health

Resources & Further Reading

Questions about evaluating mine reclamation success

Methods and activities on evaluating mine reclamation success