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Author Comment Last activity Moderation
warren "I think @gretchengehrke is back from GOSH in Switzerland, so maybe she can chime in, but I wanted to question specifically @ethanbass's point: the..." | Read more » over 8 years ago
warren "Gretchen is a chemist, so I thought she could shed some light on our questions about overlap and shorter wavelength LEDs. " | Read more » over 8 years ago
warren "@gretchengehrke, can you help us w excitation emission wavelength questions? " | Read more » over 8 years ago
ethanbass "One more thing to consider is that our DVD diffraction gratings would probably filter out most of the UV light below about 390 nm, even if we had a..." | Read more » over 8 years ago
stoft "Aha; a very good description which explains things I'd read. I think it also directly supports my present contention that with a 400nm source and t..." | Read more » over 8 years ago
ethanbass "@warren - According to the paper that figure is lifted from, you will get increasing fluorescence intensity (or quantum yield), as you increase the..." | Read more » over 8 years ago
ethanbass "@warren & @stoft - It's important to remember that the range of fluorescent emissions is determined in large part by the excitation wavelength ..." | Read more » over 8 years ago
stoft "RIght, Jeff, those are good questions and I'm not a Chemist -- I'm just extracting from what I glean from the web. But I think it would be good to ..." | Read more » over 8 years ago
stoft "https://publiclab.org/profile/ethanbass - Thanks for your comments. As to your last note about my final plot: What I see is the depth of the "notch..." | Read more » over 8 years ago
warren "Oh my goodness, I realized that I have always assumed that fluorescence emission wavelength was relative to something inherent in the chemical stru..." | Read more » over 8 years ago
stoft "Ok, good points and questions. [ I do not intend to sound abrupt. I'm just focused on raising technical issues I see within and surrounding this t..." | Read more » over 8 years ago
ethanbass "@warren - I don't think using a 395 nm LED or an even shorter wavelength is really going to solve the problem of the overlap between the light scat..." | Read more » over 8 years ago
warren "Hi, Dave - thanks as always for a thorough response. Some comments: However, the wide bandwidth of the LED, as opposed to the laser, is clearly a ..." | Read more » over 8 years ago
stoft "Jeff, I did a few quick tests and added an "Opacity" section above. While more observations are needed, these limited results are interesting. " | Read more » over 8 years ago
stoft "Thanks; good comments. 1 - Dilution: I updated the note to clarify that the larger signals were only attenuated to better match the range of the w..." | Read more » over 8 years ago
warren "I especially appreciated your introduction of a "false positive" of laundry detergent here; excellently displayed too. In your first two conclusio..." | Read more » over 8 years ago
warren "There is a LOT in this post; first, thank you Dave for a very thorough analysis! I'm sure I'll have more to add, but to start with, a clarificatio..." | Read more » over 8 years ago
eustatic "wow " | Read more » over 8 years ago
stoft "Gretchen, thanks for the insight. I'd read that the generally broad fluorescence spectrum is, as would be expected, a combination of many spectral..." | Read more » over 8 years ago
gretchengehrke "This is a really great post, Dave! As for the concentration effects, in addition to quenching, there is also shielding, and there can be another p..." | Read more » over 8 years ago
stoft "I just ran some new calculations looking at the curves from my existing data and found a possible correlation between oil density and the centroid ..." | Read more » over 8 years ago
stoft "Jeff, thanks. 1 - Yes, you are right, there is a "glow" from EVO. However, that peak is small and out at 650nm past where it has little effect. I ..." | Read more » over 8 years ago
warren " "visually differentiate between the five (5) test samples" - here, you mean based on qualitative visual inspection of the graphs, right? Do you be..." | Read more » over 8 years ago
warren "This is super, and very impressive, Dave. I have a number of comments/questions, but I'll start with just a few: I'm very surprised to hear that ..." | Read more » over 8 years ago