| Author | Comment | Last activity | Moderation | ||
|---|---|---|---|---|---|
| warren | "I think @gretchengehrke is back from GOSH in Switzerland, so maybe she can chime in, but I wanted to question specifically @ethanbass's point: the..." | Read more » | almost 9 years ago | |||
| warren | "Gretchen is a chemist, so I thought she could shed some light on our questions about overlap and shorter wavelength LEDs. " | Read more » | almost 9 years ago | |||
| warren | "@gretchengehrke, can you help us w excitation emission wavelength questions? " | Read more » | almost 9 years ago | |||
| ethanbass | "One more thing to consider is that our DVD diffraction gratings would probably filter out most of the UV light below about 390 nm, even if we had a..." | Read more » | almost 9 years ago | |||
| stoft | "Aha; a very good description which explains things I'd read. I think it also directly supports my present contention that with a 400nm source and t..." | Read more » | almost 9 years ago | |||
| ethanbass | "@warren - According to the paper that figure is lifted from, you will get increasing fluorescence intensity (or quantum yield), as you increase the..." | Read more » | almost 9 years ago | |||
| ethanbass | "@warren & @stoft - It's important to remember that the range of fluorescent emissions is determined in large part by the excitation wavelength ..." | Read more » | almost 9 years ago | |||
| stoft | "RIght, Jeff, those are good questions and I'm not a Chemist -- I'm just extracting from what I glean from the web. But I think it would be good to ..." | Read more » | almost 9 years ago | |||
| stoft | "https://publiclab.org/profile/ethanbass - Thanks for your comments. As to your last note about my final plot: What I see is the depth of the "notch..." | Read more » | almost 9 years ago | |||
| warren | "Oh my goodness, I realized that I have always assumed that fluorescence emission wavelength was relative to something inherent in the chemical stru..." | Read more » | almost 9 years ago | |||
| stoft | "Ok, good points and questions. [ I do not intend to sound abrupt. I'm just focused on raising technical issues I see within and surrounding this t..." | Read more » | almost 9 years ago | |||
| ethanbass | "@warren - I don't think using a 395 nm LED or an even shorter wavelength is really going to solve the problem of the overlap between the light scat..." | Read more » | almost 9 years ago | |||
| warren | "Hi, Dave - thanks as always for a thorough response. Some comments: However, the wide bandwidth of the LED, as opposed to the laser, is clearly a ..." | Read more » | almost 9 years ago | |||
| stoft | "Jeff, I did a few quick tests and added an "Opacity" section above. While more observations are needed, these limited results are interesting. " | Read more » | almost 9 years ago | |||
| stoft | "Thanks; good comments. 1 - Dilution: I updated the note to clarify that the larger signals were only attenuated to better match the range of the w..." | Read more » | almost 9 years ago | |||
| warren | "I especially appreciated your introduction of a "false positive" of laundry detergent here; excellently displayed too. In your first two conclusio..." | Read more » | almost 9 years ago | |||
| warren | "There is a LOT in this post; first, thank you Dave for a very thorough analysis! I'm sure I'll have more to add, but to start with, a clarificatio..." | Read more » | almost 9 years ago | |||
| eustatic | "wow " | Read more » | almost 9 years ago | |||
| stoft | "Gretchen, thanks for the insight. I'd read that the generally broad fluorescence spectrum is, as would be expected, a combination of many spectral..." | Read more » | almost 9 years ago | |||
| gretchengehrke | "This is a really great post, Dave! As for the concentration effects, in addition to quenching, there is also shielding, and there can be another p..." | Read more » | almost 9 years ago | |||
| stoft | "I just ran some new calculations looking at the curves from my existing data and found a possible correlation between oil density and the centroid ..." | Read more » | almost 9 years ago | |||
| stoft | "Jeff, thanks. 1 - Yes, you are right, there is a "glow" from EVO. However, that peak is small and out at 650nm past where it has little effect. I ..." | Read more » | almost 9 years ago | |||
| warren | " "visually differentiate between the five (5) test samples" - here, you mean based on qualitative visual inspection of the graphs, right? Do you be..." | Read more » | almost 9 years ago | |||
| warren | "This is super, and very impressive, Dave. I have a number of comments/questions, but I'll start with just a few: I'm very surprised to hear that ..." | Read more » | almost 9 years ago |