The Basic Microscope Kit is an introductory Do-It-Yourself microscope made from affordable and easy to find parts. It's based on [the Hackteria microscope](https://publiclab.org/notes/partsandcrafts/10-26-2017/making-a-hackteria-microscope), the OpenFlexure Microscope, and @maxliboiron's work at http://CivicLaboratory.nl ( #13503 ), among other sources. (above image by @partsandcrafts) ## Goals This project was started as a collaboration between Public Lab and [Parts & Crafts](http://partsandcrafts.org). The goals of the project are: 1. offer a very easy starting point for DIY microscopy 2. act as a stepping-stone to more advanced DIY microscopes 3. be as affordable and simple as possible while still providing great microscopic images 4. be good enough to view 2-5 micron size particles (see [sample images below](#Sample+images)) ##About the parts ### General parts list * a lens (objective) * a [Raspberry Pi](/tag/raspberry-pi) * a [Raspberry Pi camera](/pi-camera) * a light source * a rigid stage to connect these all together ###Example parts list for building a Basic Microscope: * [Raspberry Pi camera](https://www.amazon.com/SainSmart-Camera-Module-Webcam-Raspberry/dp/B00FGKYHXA/ref=sr_1_1_sspa?s=electronics&ie=UTF8&qid=1516378086&sr=1-1-spons&keywords=raspberry+pi+camera&psc=1) * [Raspberry Pi Zero W](https://www.amazon.com/CanaKit-Raspberry-Wireless-Starter-Official/dp/B06XJQV162/) * [a micro SD card](https://www.amazon.com/SanDisk-MicroSDHC-SDSDQM-B35A-Without-Adapter/dp/B004ZIENBA/ref=sr_1_4?ie=UTF8) * [a USB light](https://www.amazon.com/I-MU-Flexible-Gooseneck-Brightness-Gray-White/dp/B071Y3P8ZC/) * [a lens (objective x40)](http://www.amscope.com/40x-achromatic-microscope-objective.html) * [a hackteria microscope stage](https://publiclab.org/notes/partsandcrafts/01-22-2018/building-the-microscope-stage) (with modifications from Parts and Crafts!) * [a 3D printed objective adapter](https://github.com/rwb27/openflexure_microscope/blob/master/stl/optics_picam2_rms.stl) * the bolts to hold it together Extras to help you get started: * [Microscope slides](https://www.amazon.com/gp/product/B008JFRP1K/ref=od_aui_detailpages00?ie=UTF8&psc=1) * [A microscope stage calibration slide](http://www.amscope.com/microscope-stage-calibration-slide-for-usb-camera-0-01mm-stage-micrometer.html) * [Microscope Immersion Oil](https://www.amazon.com/gp/product/B007PD4IZS/ref=ox_sc_act_title_1?smid=A35BV4NHHC5J5C&psc=1) * Rubberbands * A magnet **** ## Getting started Start with the [Overview activity, here](/notes/partsandcrafts/11-26-2017/building-a-raspberry-pi-microscope). Then see [the activities below](#Activities) for each step in detail. _Basic Prototyping Kit now available for sale in the [Public Lab store](https://publiclab.myshopify.com/collections/under-development/products/basic-microscope-prototyping-kit-preorder?variant=7861722578972)!_ **** ## Questions [questions:basic-microscope]  ## Activities [activities:basic-microscope] ## Challenges We're working to refine and improve DIY spectrometry on a number of fronts; here, take a look at the leading challenges we're hoping to solve, and post your own. For now, we're using the Q&A feature, so just click "Ask a question" to post your own challenge. [questions:basic-microscope-challenge] **** ## People Folks involved in getting this project started include: [people:group:partsandcrafts] **** ## Sample images [](/system/images/photos/000/022/598/original/sample_1.jpg) [](/system/images/photos/000/022/597/original/sample_3.jpg) ...
| Author | Comment | Last activity | Moderation | ||
|---|---|---|---|---|---|
| warren | "I heard you also have a lasercutter? These new files for the stage can be cut pretty easily by hand or by lasercutter -- would you be able to send ..." | Read more » | over 6 years ago | |||
| j-bower | "Of course! Thanks for the link, and thanks Richard! " | Read more » | over 6 years ago | |||
| warren | "@j-bower, thanks for offering to help print these! " | Read more » | over 6 years ago | |||
| warren | "@j-bower -- Richard Bowman has taken a stab at a combined lens mount for v1 and v2 cameras! https://github.com/rwb27/openflexure_microscope/issues/..." | Read more » | over 6 years ago | |||
| cfastie | "If that model is modified to allow for the components and the ribbon, it should be done so the new underside surfaces are not horizontal. FDM print..." | Read more » | over 6 years ago | |||
| warren | "Although that's not the right piece -- we actually need the wider adapter; i think Richard was just saying he thought the camera interface was modi..." | Read more » | over 6 years ago | |||
| warren | "Richard from OpenFlexure has proposed this piece, although I'm not sure it's compatible with both cameras -- https://github.com/rwb27/openflexure_m..." | Read more » | over 6 years ago | |||
| Paquicamus | " richardbowman commented 5 days ago @kgradow1, thank you for your information and link. I will try and follow the directions. At the moment, I am ..." | Read more » | over 6 years ago | |||
| richardbowman | "@kgradow1, you're dead right - the pitch of the pixels on the camera module (v2) is 1.12um so the fine particles do show up as little dots. Fun fa..." | Read more » | over 6 years ago | |||
| kgradow1 | "Paquicamus, there are a number of adapters that are available through Thingiverse to connect the Raspberry Pi camera to a stereo microscope, for in..." | Read more » | over 6 years ago | |||
| kgradow1 | "That's really helpful, thank you! I feel like I need to reread and synthesize this a bit, since I still have a very rudimentary idea of how micro..." | Read more » | over 6 years ago | |||
| stevie | "Wow that's great information. Thanks for posting! " | Read more » | over 6 years ago | |||
| stevie | "Out of the two posted above, when we were testing them out, I had a preference for this one . It's currently unavailable, but perhaps they could be..." | Read more » | over 6 years ago | |||
| warren | "Also, I'd really like to give this a try as a more contained way to mount the lens -- although surely with some potential rigidity issues too... " | Read more » | over 6 years ago | |||
| warren | "hi, @dusjagr!! Still digging through all the great work on Hackteria -- have you used this design with a Raspberry Pi? @partsandcrafts @bmela @kgr..." | Read more » | over 6 years ago | |||
| richardbowman | "Hey, nice work! I thought I'd chime in with some information about tube length in case it's helpful. You're right that most RMS microscope object..." | Read more » | over 6 years ago | |||
| Bronwen | "How does the Cell Phone flashlight compare to the LED? Have the folks who have been using the microscope found that there are benefits to brighter ..." | Read more » | over 6 years ago | |||
| warren | "Just as a price reference, Thingiverse offers a kind of crowdsourced printing service and various services seem to offer about $12 as a quote for t..." | Read more » | over 6 years ago | |||
| warren | "There seems to be a small piece that sits under the camera too! http://partsandcrafts.org/pi_microscope/picam2_lens_gripper.stl That would be ama..." | Read more » | over 6 years ago | |||
| warren | "And brainstorming low-cost options at https://publiclab.org/questions/warren/12-08-2017/how-cheaply-can-i-get-all-the-parts-of-the-basic-microscope..." | Read more » | over 6 years ago | |||
| warren | "I'm not sure if these stay on when you click them, but we can probably find a similar product that does. I also wonder if we could put a diffuser i..." | Read more » | over 6 years ago | |||
| warren | "Here's a really cheap $1.50 keychain light that we could tape to something: https://www.amazon.com/Favorict-Colorful-Flashlight-Keychain-Batteries..." | Read more » | over 6 years ago | |||
| cfastie | "There is one part at Thingiverse. Is that the only one you need printed? The part at Thingiverse has a flat horizontal lower surface which could ca..." | Read more » | over 6 years ago | |||
| warren | "I posted this on Thingiverse too! https://www.thingiverse.com/thing:2820427 " | Read more » | over 6 years ago |