What I want to do
I have an openflexure microscope and I want to calibrate it. I've acquired two tools to do so, a stage micrometer, and 2.07μm Polystyrene Latex (PSL) Spheres by Ladd Research. I have previously documented the method for calibration with the stage micrometer that i learned from @AmberWise and Andrew Maselli at Chicago State, and recently replicated it with my OpenFlexure microscope.
My goal is to understand whether a stage micrometer calibration is sufficient to accurately size small particles, since the stage micrometer is reusable and fairly affordable (less than $100). After analysis, I should be able to compare the average particle size of the particles (2.07μm) to the measured particles size.
I'm going to prepare a slide with the PSL spheres, take a calibration image of the stage micrometer, and then take 40 images of the prepared slide with the microscope. then run it through @simonpyle's ImageJ macro, followed by the analysis spreadsheet @AmberWise and I put together.
time to do this!