Answers to some common questions about building, designing, & using a spectrometer:
Do I calibrate against a CFL bulb then switch to a halogen bulb for taking spectrums?
Yes, the CFL is only for wavelength (spectral) calibration, not intensity. So you can do it often just in case your device is "drifting" but it's not necessary for different containers or sample types.
Are the spectrums taken by different people really comparable?
What if everyone has different setups from light source to model of camera to manufacturer of the DVD used for the defraction grate?
We need to figure this out. One reason to do a big kit order as in the Kickstarter is to give everyone the same hardware. But I hope that as we get better at exposure calibration this will be less of a problem.
Can you use a still camera with Spectral Workbench instead of a webcam?
Yes but you have to manually upload the image, and sometimes crop it too. It's just slower, but you can then do long exposures, which makes for MUCH better sensitivity.
What camera should I use to build my own?
I have a Logitech 640x480 webcam -- should I upgrade to a 720p or 1080p webcam?
Any USB webcam will do -- HD is preferable and the higher resolution the better, but a good quality 640x480 is usable. We use SYBA brand HD webcams in the kits. Bigger sensors are better, as are ones good in low-light.
Why a dimable light? Doesn't that mess up quantitative results?
Some samples need a higher amount of light because they're just too opaque. We definitely need a way to quantify this. I was thinking something like HDR photography, or something. Mainly it's challenging because the webcams often do auto-exposure compensation and we have to disable that.
UV -- is this a better method than visible light?
Do you see clearer lines? Do materials absorb UV "better" than other wavelengths? Is there some particle-level difference in how light is absorbed?
The difference is that for poly aromatic hydrocarbons (and some other contaminants) there isn't a lot of well-known spectral identifying features (like absorbance lines) in the visible or near-infrared range. There's good literature about fluorescence spectroscopy, though, which is where UV light excites the material to actually glow, sometimes in the visible range. So for oil contamination, we're focusing on UV-fluorescence instead of visible light or infrared spectroscopy. Read more here: http://publiclaboratory.org/wiki/uv-spectrometry
Reflectance vs. absorption -- what's the difference?
When light reflects off a material and into your spectrometer, it only interacts with the top layer of atoms or so. With transmission spectroscopy, light passes through the material, interacting with anywhere between a few microns and (with a liquid sample) a few centimeters of material before entering your instrument. There's just more sample to absorb light.
Is "intensity" just the same as "brightness"?
I.e. what do we see from looking at the peaks that we don't see from just looking at the image itself?
The graph of intensity is easier to compare against another spectrum, as it's quantitative. It's also turning your perception of brightness into some numbers which you could use to automatically match with another spectrum from our library of spectra. But sometimes I do feel like you can visually pick out bright or dark lines with the naked eye much better.
Why do some spectra have distinct lines, and some don't?
I think what I'm supposed to be looking for are absorption lines -- but it seems like with spectra of many materials it's hard to get the kind of clear lines you get with gas tubes (say, in pictures on the internet). If not lines, what's useful to see here?
This is where the graphs can help. If you can see a generally brighter region between a sample and control spectra, there's a broadband absorption. But that may not carry a lot of very specific information. With the pancetta samples, we could say, it's just blocking a lot of UV. Meaning it'd be a good sunscreen ;-)
When I see dark lines in the CFL spectra, am I seeing absorption lines?
Or am I just seeing the lack of emitted wavelengths? (and if so, am I looking at "emission lines" as the converse of "absorption lines," and does that even make sense when I'm looking at a full spectrum light?)
The CFL spectra are actually from fluorescence, hence "fluorescent light". So you're looking at the brightness lines where light is produced, not the missing dark lines where it's absorbed. Yes, you're right, it's kind of the inverse.
The blue LED in the Desktop kit is interfering with the image. What can I do?
Use a left over piece of the black paper to make a partition between the LED and the lens. Cut it to size and friction will keep it in place.
I can't turn the lens, what gives?
Make sure you are grabbing the lens at the top. There is a serrated ring at the base of the camera, which looks like it might turn. It does not. That is not what you want. The lens itself is above that.
Unanswered questions -- aka research challenges!
If you can answer 'em, move them up to the top section!