Spectrometry
question:spectrometry

The Public Lab spectrometry project is an open source community effort to develop low-cost spectrometers for a range of purposes. All open spectrometry hardware and software efforts are welcome here! **Join in by:** * Learning [what spectrometry is](#Whats+spectrometry) * Reading about goals and [asking great questions](#Frequently+Asked+Questions) * Building a basic spectrometer using [one of our starter kits](#Starter+Kits) * Trying (and critiquing) our [community-made how-to guides](#Activities) and posting your own * Building on others’ work; hack and remix the kits to refine and expand them * [Share your upgrades](#Upgrades) for others to try -- and perhaps for inclusion in an upcoming starter kit release or add-on kit **** ## Starter Kits Public Lab’s Kits initiative offers several starter kits, including many of the basic components, and instructions for constructing a basic visible light spectrometer. The point of the kits is to provide a shared reference design for building experimental setups onto. Lego Spectrometer Kit Our most recent kit, incorporating community improvements while balancing low cost and ease of construction. Choose between webcam and Raspberry Pi camera versions and build attachments width standard Lego connectors. Build one Buy one Papercraft Spectrometry Intro Kit A $9 paper spectrometer which you can attach to a smartphone or webcam. It’s made of paper to reduce cost and complexity, and is mainly intended as an “introductory” or educational kit. The flat design can be printed on a laser printer or photocopied to make more. Build one Buy one **** ## Activities This is a list of community-generated guides for specific applications using your spectrometry setup (either a [starter kit](#Starter+Kits) or a [modded design](#Upgrades)). These [activities can be categorized](https://publiclab.org/wiki/activity-categories), and some may be more reproduced -- or reproducible -- than others. Try them out to build your skills, and help improve them by leaving comments. Together, we can repeat and refine the activities into experiments. > **Note:** If you are working on an **urgent issue** such as a threat to your or someone else’s health, please know that these techniques may not be ready for your use; it's possible that they never will be. [Read more here](/notes/gretchengehrke/09-29-2016/common-low-cost-technique-limitations) ### Activity grid [activities:spectrometry] **** ## Upgrades Have you added to your starter kit, improved it, or redesigned it? Show others how to take it to the next level by posting a build guide here: [upgrades:spectrometry] Add your upgrade guide here Request or propose an upgrade _Mods should include a parts list and a step-by-step construction guide with photo documentation. See an example._ **** ## Challenges We're working to refine and improve DIY spectrometry on a number of fronts; here, take a look at the leading challenges we're hoping to solve, and post your own. For now, we're using the Q&A feature, so just click "Ask a question" to post your own challenge. Be sure to add: * constraints: expense, complexity * goals: performance, use cases [questions:spectrometry-challenge] **** ## Builds There’s a lot going on in open source spectrometry -- if you’ve developed another open source design you’d like to show others how to construct, post it here! * [RamanPi](https://hackaday.io/project/1279-ramanpi-raman-spectrometer) * [Hackteria “drop”-style spectrometers](https://publiclab.org/notes/gaudi/04-03-2014/diy-micro-volume-spectrophotometer) / [DIY NanoDrop on Hackteria.org](http://hackteria.org/wiki/index.php/DIY_NanoDrop) * _Add yours here_ ##What's spectrometry? Colored light is often a blend of different colors. A spectrometer is a device which splits those colors apart, like a prism, and measures the strength of each color. A typical output of a spectrometer looks like this spectrum of the daytime sky, with the actual light spectrum at the top and the graph of wavelength (horizontal axis, in nanometers of wavelength) and intensity (vertical axis) below: [![sky.png](https://i.publiclab.org/system/images/photos/000/005/455/original/sky.png)](https://spectralworkbench.org/analyze/spectrum/19882) > Needed: overview of spectra, calibration, units, comparison, and fluorescence/absorption. Please edit this page or link to a resource, potentially [the Wikipedia page](https://en.wikipedia.org/wiki/Spectroscopy), although that's quite full of technical jargon. ## Software Spectral data can be analyzed with https://spectralworkbench.org to create spectra plots, find centers of emissions plots, and find similar spectra. Data also can be exported in various formats (JSON, CSV, XML) for further analysis and visualization. ## How does this compare to a lab instrument? The [Desktop Spectrometry Starter Kit](/wiki/desktop-spectrometry-kit-3-0) is only one part in an experimental setup, and the following shows where it fits in an overall diagram of a lab spectrometric setup: [![tmp_31873-IMG_20161027_101601_2-79757779.jpg](https://publiclab.org/system/images/photos/000/018/635/large/tmp_31873-IMG_20161027_101601_2-79757779.jpg)](https://publiclab.org/system/images/photos/000/018/635/original/tmp_31873-IMG_20161027_101601_2-79757779.jpg) [![tmp_31873-IMG_20161027_095939_2-108076392.jpg](https://publiclab.org/system/images/photos/000/018/636/large/tmp_31873-IMG_20161027_095939_2-108076392.jpg)](https://publiclab.org/system/images/photos/000/018/636/original/tmp_31873-IMG_20161027_095939_2-108076392.jpg) There are many, many different types of spectrometry and spectrometers -- many don’t even measure light. Even among those that do, some detect light in the ultraviolet range, and others in the infrared range of light. The range of Public Lab spectrometers depends on the range of the commercially available cameras we attach them to (~400-700 nanometer wavelengths). A commercially available product with a slightly wider range (from 335 to 1000 nanometers) is [available from Cole Parmer](http://www.coleparmer.com/Product/Cole_Parmer_Visible_spectrophotometer_335_to_1000_nm_wavelength_range_analog_output/UX-83055-10). **** ## Frequently Asked Questions [questions:spectrometry] Note our previous Frequently Asked Questions page, which [can be found here »](/wiki/spectrometer-faq) -- please help port these into the new system, here!...


Author Comment Last activity Moderation
vivek14 "hello " | Read more » almost 2 years ago
vivek14 " hello there is no iphone app . sir your question is very useful for all .I am just looking khanapara teer . " | Read more » almost 2 years ago
Ag8n "Now, to change the topic a little bit. What happens once all this testing gets going? In industry, a financial auditor appears and says " you are..." | Read more » about 2 years ago
Ag8n "There are variations on the method, but check out the USP if using for medical applications. The oldest is a straight " gel clot". The sample is ..." | Read more » about 2 years ago
Ag8n ""Pharmacopeia online" is at " uspbpep.com". " | Read more » about 2 years ago
Ag8n "The Wikipedia article is an interesting read. The initial data said horseshoe crab mortality from L.A.L. testing should be about 3%. However, rec..." | Read more » about 2 years ago
Ag8n "Here's where life gets a bit interesting. There are substances called pyrogens. These cause a fever, sometimes severe, even if the carrier bacteri..." | Read more » about 2 years ago
Ag8n "As a refresher, read USP 31, <1231>"Water for Pharmaceutical Purposes". This is available, for free, from Pharmacopeia online, as mentioned ..." | Read more » about 2 years ago
Ag8n "Error here. It should not be objective c, as noted above. It should be some plastics are showing higher extractables than bulk water. Something..." | Read more » over 2 years ago
Ag8n "Getting a hold of the USP used to be difficult and expensive. While current USP may still be expensive, here is a source for older versions. "Pha..." | Read more » over 2 years ago
Ag8n "USP/BP/EP means United States Pharmacopoeia,BP is British Pharmacopoeia, and EP is European Pharmacopoeia. Sections of the USP are divided into mo..." | Read more » over 2 years ago
Ag8n "The CO2 produced can be measured several ways. Each method has advantages and disadvantages and depends on the final use of the instrument. For exa..." | Read more » over 2 years ago
Ag8n "If you want an overview of TOC, Wikipedia is a good start. The current article seems pretty thorough ( dated 19 July 2022 by 75.143.92.215). Any c..." | Read more » over 2 years ago
Ag8n "Previous methods could be somewhat hazardous. To give an example, ammonia related chemicals often work their way into drug compounds. It can be f..." | Read more » over 2 years ago
Ag8n "Going back over this. We did a lot of work with total organic carbon (TOC). According to Dr. Paul Whitehead at egalabwater.com (the blog title is..." | Read more » over 2 years ago
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irganga "A tip is to put the DVD on a pan with water and boil it for 5 to 10 minutes. It would be easier to separate the layers with a knife. " | Read more » over 2 years ago
smyckeskrin "i don't understand your question " | Read more » over 2 years ago
bhamster "Hi @Cheesealmighty, thanks for sharing about your work. While it's not spectrometry, there are a couple research notes on turbidity sensors by @wma..." | Read more » over 2 years ago
Cheesealmighty "We won't be making any changes to the catheter itself, it's not much different from taking photos of the see through tube/collection chamber. It sh..." | Read more » over 2 years ago
Ag8n "Has Medtronic been approached about this? That should probably be the first approach. As far as the rest of it goes... We made catheters, heart a..." | Read more » almost 3 years ago
Ag8n "On battery life- it wasn't very good. Mercury (as in mercury lamps) have a lot of lines that go all through the uv/vis spectrum. For a while(as in ..." | Read more » almost 3 years ago
warren "Definitely these can be dangerous for the eyes. The LEDs seem nice because they are a little less 1800s feeling than those tiny tiny mercury bulbs!..." | Read more » almost 3 years ago
Ag8n "I got one of the 254 nm UVC lamps that might be usable for an uv/vis instrument as a gift . It's advertised as for sterilization of surfaces. The ..." | Read more » almost 3 years ago