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Question:Fluorescence microscope - automatically detect airborne pathogens in confined space?

vwaterson asked on December 26, 2017 19:21
108 | 0 answers | shortlink


Need to detect pathogen levels in a 240 cu ft room after it has been subjected to dry fogging using a diluted hydrogen peroxide mixture.



fluorescence microscopes pathogens

question:general



7 Comments

warren 22 days ago

Hi, can you fill us in a bit more on details of your interest and situation? What kinds of pathogens, and what is dry fogging? Sounds interesting!

vwaterson 22 days ago

Dry fog technology utilizes a chemical disinfection process: the system vaporizes the sporicidal chemical, producing very fine droplets of disinfectant which are dispersed throughout a room, without any manual efforts. ... The dry fog solution is evenly and completely dispersed in the room or rooms. We need to be able to determine that the CFU (n microbiology, a colony-forming unit (CFU, cfu, Cfu) is a unit used to estimate the number of viable bacteria or fungal cells in a sample. Viable is defined as the ability to multiply via binary fission under the controlled conditions. ... Expressing results as colony-forming units reflects this uncertainty.) has been reduced to predetermined level of either 3-Log or 6-Log reduction i.e. 99.9% of 99.9999%

warren 22 days ago

Ah, ok -- do you have example images of the kinds of data you'd need to collect, and a pixel/micron resolution you're aiming for?

Also, what kind of fluorescent light would you need?

warren 22 days ago

I'm thinking of whether the Basic Microscope Kit could be helpful:

http://publiclab.org/basic-microscope

and also something like this LED light: https://publiclab.org/notes/warren/03-10-2016/honlian-60s-led-nail-polish-hardener-various-led-fluorescence-updates

vwaterson 22 days ago

The basis microscope kit and LED light are interesting but I would also need software which could automatically analyse the results as we cannot have any human intervention

warren 22 days ago

Interesting! Do you have an example to refer to in how an automatic analysis could work?

vwaterson 22 days ago

Sorry no I don't have an example.

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