I need the help of a microbiologist. L.a.l. or lysate testing is often used to test for pyrogens. That is microbes that can produce a reference if dead. It often used an extract of horseshoe crab blood to do this.
The test can be done visually, via turbidity, or with a spectrometer. The spectrometer used 405 nm, which is pushing it for the public labs spectrometer.
What special equipment, testing, etc.,is needed for the microbiological end of the test?