I need the help of a microbiologist. L.a.l. or lysate testing is often used to test for pyrogens. That is microbes that can produce a reference if dead. It often used an extract of horseshoe crab blood to do this.
The test can be done visually, via turbidity, or with a spectrometer. The spectrometer used 405 nm, which is pushing it for the public labs spectrometer.
What special equipment, testing, etc.,is needed for the microbiological end of the test?
Thank you.
A partial answer. Each block of analyzers is heated to 37.0 C . They do not use a spectrometer. Instead, each cell in the analyzers has a led (at 405 nm) and photocell attached. There are 96 cells in the block that run at the same time, so the test takes some time.
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