Question: Can the spectrometer or turbocharger be used for lal testing?

Ag8n is asking a question about spectrometry-challenge: Subscribe to answer questions on this topic

Ag8n asked on April 02, 2018 00:32
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I need the help of a microbiologist. L.a.l. or lysate testing is often used to test for pyrogens. That is microbes that can produce a reference if dead. It often used an extract of horseshoe crab blood to do this.

The test can be done visually, via turbidity, or with a spectrometer. The spectrometer used 405 nm, which is pushing it for the public labs spectrometer.

What special equipment, testing, etc.,is needed for the microbiological end of the test?

Thank you.

spectrometer spectrometry microbes turbidity spectrometry-challenge horseshoe-crab lysate pyrogens

question:spectrometry-challenge


2 Comments

  
stevie 3 months ago

Woah interesting question! can you give me some context or background for it? I'll volunteer to do some hunting for someone who can help answer :)

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Ag8n 3 months ago

Thanks! Give me a couple of days and I'll email you a more detailed description. The help is appreciated!


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1 Answers

Ag8n 3 months ago

A partial answer. Each block of analyzers is heated to 37.0 C . They do not use a spectrometer. Instead, each cell in the analyzers has a led (at 405 nm) and photocell attached. There are 96 cells in the block that run at the same time, so the test takes some time.

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