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Oil testing kit Beta programme - Calibration notes

by cindy_excites | October 31, 2015 22:15 | 166 views | 6 comments | #12349 | 166 views | 6 comments | #12349 31 Oct 22:15

Read more: publiclab.org/n/12349


Oil testing kit Beta programme - Calibration notes

Contributors: @TedF and @cindy_excites

Date: 31/Oct/2015

Here we document our experience using Spectral workbench to calibrate the Public Lab Spectrometer 3.0, which we assembled using the instructions provided there. We also put forth a few humble suggestions, including a "for dummies" outline of our steps.

1) Aligning the 'spectrum' When first trying to capture spectra, we noticed the line was not straight. We opened the spectrometer's black cardboard frame to tweak the DVD fragment and improved the angle:

Crooked_spectra.png

Still not satisfied with the angle of the refracted line, we reopened the spectrometer to adjust the angle of the DVD fragment:

Aligned_spectra.png

This is what it looks like on the inside:

IMG_4125.JPG

(I admit that since I did not understand why the DVD fragment didn't have to cover the whole of the 'mid hole' of the 'diffraction grating angle' I covered the whole thing. Maybe it will come back and nip us in the butt! However, I did this because as you will read below, I had qualms about the DVD fragment's quality due to unsatisfactory separation. If the quality of the DVD fragment turns out to be problematic, I'll try @ethanbass's recommendation with MrBumper's method).

Success! We used the lamp from a phone - shining it directly into the slit - to check this. At first I thought this misalignment was due to the DVD not having been peeled/separated properly:

IMG_4122.JPG

Does it matter if it still refracts the light? What is good enough for a DVD fragment? Here is our piece of DVD:

IMG_4120.JPG

2) Working with Spectral workbench…

Spectral_workbench_calibration_success.png

a) What is that little red lightbulb telling me??

Spectral_workbench_-_red_lightbulb_crp.jpg

When trying to capture the light of a CFL lightbulb, we noticed that that the 'Detected fluorescent light' bulb symbol turned red. We searched the website for guidance on this but couldn't immediately find anything…). We assumed that it meant that fluorescent light had been detected… although it did not always turn red when capturing our diffused light… Does it warn for overexposure instead…?

The youtube video on calibration on the 'Spectral workbench usage' page is very easy to follow even though it features the previous version of Spectral workbench. However, I'm wondering if for the absolute beginner, it might be useful to have visual guide: a screenshot of the new Spectral workbench interface with little arrows pointing to what the different buttons do.

b) Figuring out how/when to capture spectra…

It took us a little while to get acquainted with the site; we did not really know what exact steps to follow and without background knowledge or points of reference. However, in the end we made it and decided to create a "Calibration for dummies" version of our steps. Might be useful for the absolute beginner :O

i) We arrive at Spectral workbench using the Firefox browser (seems to work better than Chrome). We share our camera labelled "USB 2.0 Camera" as opposed to the integrated camera:

Spectral_workbench_-_camera_sharing_menu.png

Spectral_workbench_-_camera_sharing.png

ii) We shine light from a CFL bulb at the spectrometer's slit. We see the live image on the top left and the video row on the centre screen. We click "Begin capturing"

iii) With a steady 'flow' of light into the spectrometer, we get continuous rows of colour bands on the central spectral field. When happy with the output of this, we click the blue "Save" button on the left:

Spectral_workbench_-_successful_spectral_capture_using_plastic_bag.png

We receive message "Spectrum was successfully created". Yey!

NOTE: Our original capture returned an error message "You must be logged in". We repeated the procedure and we received an error message warning that our spectrum is overexposed. We click the link to "how to fix this" [ http://publiclab.org/wiki/spectral-workbench-usage#Overexposure ]. We opt for using a diffuser - a plastic bag (a clear one, not a milky one):

IMG_4127.JPG

iv) We then proceed to calibrate by clicking the blue "Calibrate" button on the bottom left.

c) Calibration procedure

Step 1: A pop-up appears instructing us to select the middle blue band of our spectrum; we click "Begin":

Calibration_procedure_1of5.png

Step 2: We move the cursor and place it on the peak of the curve for the middle blue band as instructed:

Calibration_procedure_2of5.png

Step 3: Once selected, a next pop-up appears instructing us to select the green band. We click "Finish":

Calibration_procedure_3of5.png

Step 4: We move the cursor and place it on the peak of the curve for the green band as instructed.

NOTE: The second pop-up confused us at first: we were not ready to "finish" as we had not selected our green band. This prompted us to click outside the box only to realise that we'd lost the calibration. In terms of usability, it might be best to have the blue button say "Next" and then a third pop-up saying "Finish", which then leads to the appearance of the "Great! Calibrated" message at the top of the page:

Calibration_procedure_4of5.png

Calibration_procedure_5of5_great_calibrated.png

Step 5: optional Out of curiosity we clicked on the "Find similar" button - fifth to the right of the "Calibrate" button and got this:

Calibration_procedure_-_closest_match.png

We also clicked on "Compare" - fourth to the right of the "Calibrate" button:

Calibration_procedure_-_compare.png

Calibration_procedure_-_compare_output.png

d) Next steps

Now (we suppose) we can select our best calibration capture when testing spectra from our cuvette:

Calibration_-_next_steps_1.png

Calibration_-_next_steps_2.png

Calibration_-_next_steps_3.png

Other research notes in this series:

Oil testing kit Beta programme - Package content

Oil testing kit Beta programme - Assembly: cuvette frame

Oil testing kit Beta programme - Assembly: spectrometer


6 Comments

Hi, in regards to your question about the placement of the DVD fragment, I think the idea is to align your camera so that it is pointed as close to the outer edge of the DVD diffraction grating as possible. The edge of the DVD, where the arc of the spiral is widest, will have the least curvature, so it should yield a straighter spectrum.

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Ah, yes, that makes a lot of sense, thank you Ethan! I used the part which seemed to have the least purple residue - leftover from the disc separation. Weighing the benefits of each...

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Huh, did your diffuser make it easier to get bright spectra? Why did you choose to use one?

The red light bulb is an experimental "fluorescent light bulb detector" -- it says so if you hover over it.

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Screenshot_2015-11-05_at_9.53.15_AM.png

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Cindy this is awesome :) I'm working on a workshop where participants will need to calibrate. This material is really helpful! Thanks for posting!

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@Cindy_ExCites :) This was awesome. I'm writing a workshop on calibration and scanning and this was Super helpful :). Any chance you've moved on to test the samples yet?

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