Public Lab Research note

Oil fluorescence scanning on an Ocean Optics spectrometer

by warren | September 23, 2014 17:42 23 Sep 17:42 | #11176 | #11176

What I want to do

At LEAFFEST 2014, Mary, Luisa, and others reproduced the kind of fluorescence spectrometry scanning we've been doing with the Oil Testing Kit using a lab spectrometer, an Ocean Optics device. We used the same 405nm laser pointer and the same samples -- of suspected BP crude, fish oil, and mail-ordered crude from ONTA, all dissolved in mineral oil, and plain mineral oil. Here are the graph and the files, which Louisa emailed to me:


Update: Mary sent me specs on the device, below:

Spectra were measured with an OceanOptics SD2000 dual spectrometer (, using the first spectrometer (wavelength range: 200-850nm; grating: 600 lines at 300nm; 25um slit).

Spectrometer is spectrally calibrated with a mercury/argon calibration source ( Values are relative DN, and are not radiometrically calibrated.

I had to add ".txt" to the end of each file to get them to upload, but that also makes them easier to open up.





Questions and next steps

Presumably the calibration is OK? And the intensities are adjusted -- linear -- unlike our own spectrometer. So it's could be a basis for calibrating our device, if we assume that the setup is similar enough.

I'm especially interested in importing this data into Spectral Workbench, and am working on a way to upload this format. For now, you can view one of the files on Github too; they're just a column of wavelengths, a column of intensities, and an index column counting from 0 upwards:


Hi, check this out -- @hynae !

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"Kids, don't try this at home" (without eye protection ...)

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