Hello everybody, I am a student in biochemistry at the University of Liège in Belgium, and I have a huge scientific curiosity so I decided to build my own spectrometer, with the help of Spectral Workbench informations of course, in order to analyse the spectrum of many many samples. I realised many tests, and I decided to focus my "work" on concrete things.
I think that is important to clarify that is my first research note and that I speak French, so I am sorry for the possible mistakes of English language.
I will present you the spectrum of grass extract. To do that, I picked some grass in my garden, I add water and press the grass to extract ... chloroplasts I presume :D This is my "stock solution".
So, I analysed my stock solution in a dilution 8X. I used water for the blank and I used a LED RGB but i lighted only with a blue light. I collected results and analysed them. Here is a graph with the spectrum of the blank, the sample and the difference of both (it is absorbance) :
We observe 2 maximums of absorbance, at wavelength corresponding of maximum of absorbance of chlorophyll A (~430 nm) and chlorophyll B (~445 nm).
The spectrum is not very accurate and the resolution is weak, so it is a good idea to improve my spectrometer to increase the resolution and accuracy of my future spectrum. I realised the same experiment with a red light, but results are not enough usable.
I think that my results are significant, and that stimulate myself to continue and perform a lot of spectrum of many samples.