Public Lab Research note

The user 'dhaffnersr' has been placed in moderation and will not be able to respond to comments.
  • 0

Absorption spectra of extra virgin olive oil-Supplementation part III

by dhaffnersr |

Supplemental analysis of initial absorption spectrum, related to Dave Stoft's challenge: Chemical analysis of Extra Virgin Olive Oil sample SP07-C0[4] (Chlorophyll [A] and Chlorophyll [B].

Sample preparation was done with 1 plastic disposable UV cuvette (220nm - 2200nm) and filled to a volume of 2ml of Ethyl Acetate for my reference spectrum (solvent.)

Using a Solux 4700K 12vdc lamp as my radiant light source, I acquired my first spectrum for absorption data analysis. Next, using the same cuvette, I transfered 1% extra virgin olive oil (Kirkland brand) into the cuvette and used a stainless steel cuvette mixing stick to vortex the sample.

This was my second spectral capture for my absorption study. Following procedures detailed in my previous literature, I used Spekwin32 to process and capture all data. Using the same diluted sample of Evoo (extra virgin olive oil,) I then used a 405nm UV laser pointer to capture the emission spectra.

My results and final data analysis are presented below:






[3] - excitation at 280-330 nm and emission at 372-480 nm.

spectrometer spectrum-matching spectrography image-compositing image-processing citizen-science field-test spectralworkbench grassroots-science weird-science plots ultraviolet database compact-fluorescent testing spectral-analysis tests led chlorophyll metadata data-collection data popular-science museum-of-science leds quantitative datalogger civic-science slit-width specification research-notes electrochemical wavelength technique purelab reference hopewell city-point city-point-hopewell-va dhaffnersr david-h-haffner-sr spekwin32 david-h-haffner haffner david ethanol quartz graphs gnu-plot 400nm 400-nm-led technical technical-specs technical-specifications testing-uv-led images-led-testing images-led-testing-400-nm-uv images-test-led-400nm-ultra-violet uv-spectrum-200-400-nm-leds-testing-test-results quartz-cuvettes quartz-cuvette 400nm-led-thru-quartz-cuvette-test electrical-engineering google-search meta-tag meta-tags chlorophyll-testing cool-stuff very-cool-stuff relevant uv-vis uv-vis-ethanol b aspirin uv-vis-chlorophyll dye dyed eosin eosin-y extract portable-laser emission-wavelength wavelengths generic-laser uv-vis-sodium-hydroxide redox distance-of-slit bandwidth methylene-blue blank-distilled-water aries emission-wavelength-for-rhodamine-b image-raman-spectrometer google-image-rhodamine-b-samples google-image-raman-spectrometer-homemade plots-spectrometry optical-spectroscopy circular-dichroism-spectroscopy fluorescence-dye photophysik led-testing led-test


You must be logged in to comment.