Supplemental analysis of initial absorption spectrum, related to Dave Stoft's challenge: Chemical analysis of Extra Virgin Olive Oil sample SP07-C0 (Chlorophyll [A] and Chlorophyll [B].
Sample preparation was done with 1 plastic disposable UV cuvette (220nm - 2200nm) and filled to a volume of 2ml of Ethyl Acetate for my reference spectrum (solvent.)
Using a Solux 4700K 12vdc lamp as my radiant light source, I acquired my first spectrum for absorption data analysis. Next, using the same cuvette, I transfered 1% extra virgin olive oil (Kirkland brand) into the cuvette and used a stainless steel cuvette mixing stick to vortex the sample.
This was my second spectral capture for my absorption study. Following procedures detailed in my previous literature, I used Spekwin32 to process and capture all data. Using the same diluted sample of Evoo (extra virgin olive oil,) I then used a 405nm UV laser pointer to capture the emission spectra.
My results and final data analysis are presented below:
http://cdn.intechopen.com/pdfs/27027/InTech-Analysis_of_olive_oils_by_fluorescence_spectroscopy_methods_and_applications.pdf - excitation at 280-330 nm and emission at 372-480 nm.