April 27, 2023 18:09 / Last edited by armanlybun about 1 year ago
Purification of a recombinant human growth hormone by an integrated IMAC procedure In this study, integration of three discrete process aspects of the IMAC purification of Escherichia coli expressed recombinant proteins has been investigated.
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The purification of biological active compounds such as human growth hormone from complex cell culture samples is a crucial step in proteomic research and down-stream processing of biotechnological products. Purified hGH was highly pure on silver-stained gel and contained very low levels of endotoxin. On average, ∼37 mg, ∼12 mg, and ∼7 mg of hGH were obtained from 500 mL-cell cultures of Trx-hGH, MBP-hGH, and PDIb'a'-hGH, respectively. Subsequently, hGH was analyzed using mass spectroscopy to confirm the presence of two intra-molecular disulfide bonds.
Complete solubilization and purification of recombinant human growth hormone produced in Escherichia coli High-level expression of recombinant human growth hormone (hGH) in Escherichia coli (E. coli) leads to the formation of insoluble aggregates as inclusion bodies devoid of biological activity.
Human growth hormone (hGH) was one of the first recombinant proteins approved for the treatment of human growth disorders. Its small size (191 amino acids), possession of only 2 disulphide bonds and absence of posttranslational modifications make Escherichia coli the host of choice for its production on any scale.
Aug 16, 2021
Optimization of a recombinant human growth hormone purification process using quality by design Optimization of a recombinant human growth hormone purification process using quality by design Authors
Human growth hormone (hGH) is a single-chain polypeptide containing 191 amino acid residues and is synthesized in the pituitary gland. It is one of the most important hormones in the human body due to its pivotal role in a variety of biological functions, including cell proliferation and metabolism [1].
Growth and Purification of Human Growth Hormone 1 Presenter Info: Nathan Jones Cary, NC Senior Physics Major at North Carolina State University 2 What is Human Growth Hormone ( hGH )? Protein for pharmaceutical interest 191 Amino Acids Mw 22KD Alpha-helixes mostly Study stability in different environments Use neutron scattering 3
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